Multiphoton imaging references
Two-photon fluorescence absorption and emission spectra of dyes relevant for
cell imaging.
J Microsc. 2002 Nov;208(Pt 2):108-15
Bestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T,
Berchner-Pfannschmidt U, Wotzlaw C, Acker H.
Biomedizinische Strukturforschung (A0602), Deutsches Krebsforschungszentrum, PO
Box 101949, D-69009 Heidelberg, Germany.
Two-photon absorption and emission spectra for fluorophores relevant in cell
imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable
optical parametric amplifier for the excitation range 580-1150 nm and an optical
multichannel analyser. The measurements included DNA stains, fluorescent dyes
coupled to antibodies as well as organelle trackers, e.g. Alexa and Bodipy dyes,
Cy2, Cy3, DAPI, Hoechst 33342, propidium iodide, FITC and rhodamine. In
accordance with the two-photon excitation theory, the majority of the
investigated fluorochromes did not reveal significant discrepancies between the
two-photon and the one-photon emission spectra. However, a blue-shift of the
absorption maxima ranging from a few nanometres up to considerably differing
courses of the spectrum was found for most fluorochromes. The potential of
non-linear laser scanning fluorescence microscopy is demonstrated here by
visualizing multiple intracellular structures in living cells. Combined with 3D
reconstruction techniques, this approach gives a deeper insight into the spatial
relationships of subcellular organelles.
More references (review in progress):
1 - 2 - 3 - 4 - 5 - 6 - 7 - 8 - 9 - 10 - 11 - 12 - 13
Del Mar Photonics connection:
Trestles Femtosecond Ti:Sapphire
laser
Trestles Finesse Ti:Sapphire oscillator
Mavericks Femtosecond Cr:Forsterite
laser (around 1250 nm)
Multiphoton Imaging with Mavericks -
introductory brochure
Focus on Microscopy 2007
abstract - Cr:Forsterite crystal
Business Wire: Del Mar Photonics Announces New
Mavericks (TM) Long Wavelength Femtosecond Laser for Scientific Research
and
Enhanced Biological Imaging.
High Resolution Impedance and
Potential Imaging using a Mavericks Cr:Forsterite Laser
Tamarack Femtosecond Er-doped fiber laser
(around 1560 nm)
Buccaneer Er-doped Femtosecond OA Fiber Laser
(1560nm)
Buccaneer SHG (780nm) femtosecond fiber lasers
SHG unit for femtosecond fiber lasers
Cannon Ultra-Broadband Light Source
Femtosecond Fiber Lasers from Del
Mar Photonics - Er
- Yb (pdf)