Del Mar Photonics - Newsletter Fall 2010 - Newsletter Winter 2010

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Photonics West 2011: Presentations on bimolecular fluorescence complementation

An orange fluorescent protein mKO? for bimolecular fluorescence complementation

Paper 7900-21 of Conference 7900
Date: Monday, 24 January 2011
Time: 5:30 PM

Author(s): Ting Su, Zhihong Zhang, Shaoqun Zeng, Qingming Luo, Britton Chance Ctr. for Biomedical Photonics (China)
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The bimolecular fluorescence complementation (BiFC) imaging technology based on fluorescent protein (FP) has been successfully applied in visualizing protein-protein interactions (PPI) in many cell types and organisms. To data, several fluorescent proteins with distinct spectra characteristic and from different origin have been developed to be used in BiFC technology. Now, blue (cerulean), green (EGFP), yellow (Citrine and Venus), red (mCherry) and far-red FP (mLumin) are available for BiFC application. Recently, a newly developed orange florescent protein named mKO? has been reported. Here, we identify mKO? can be used in BiFC application below 30?. To demonstrate the use of mKO? based BiFC, we successfully visualize protein-protein interaction in low organism c.elegan. Our mKO? based BiFC system testify the feasibility of orange fluorescent protein in BiFC use and fill the color gap between yellow and red spectrum.