Del Mar Photonics - DNA imaging with AFM Heron
AFM HERON was presented at Photonics West 2009 exhibition
Del Mar Photonics
AFM HERON - sample quotes and specs
|Del Mar Photonics at Photonics West 2009 - few moments from
the show floor
For the recent years Scanning Probe Microscopy (SPM) has
proved to be the extremely successful technique for characterization of
different nano-sized objects. SPM plays the important role in the outburst of
scientific and commercial activities defined by the term “nanotechnology”. As a
great number of applications for the SPM-based research are becoming more and
more diverse and complex, new challenges and demands arise for the SPM
instrumentation. Unfortunately, still the adjustment of the most modern SPMs
is complicated, time consuming and very much operator-dependant. This drawback
leads to poor reproducibility of the instrument settings and consequently to
poorly reproducible results.
New, fully motorized AFM HERON (HERO of Nanotechnology) which allows to perfectly
align a cantilever, laser and photodiode by just one click on a command button. The scanning
settings and landing parameters are also automated that allows to avoid any time consuming
adjustment operations, thus leaving more time to researcher for designing the experiment and
performing more accurate measurements.
Figure 1. Automated, one-click laser-to-cantilever alignment.
Figure 2. Distribution map of the cantilever's oscillation at the fundamental (left) and second (right) modes.
Another unique feature of AFM HERON is the
capability to map the distribution of the oscillation amplitude along the
cantilever. After the map is acquired, operator can choose the most
appropriate position of the laser spot on the cantilever for his
measurements. This becomes especially important when a cantilever is excited
at higher modes such as in the Figure 2.
The complete automation of the AFM HERON setup allows researchers to avoid time consuming and tiresome routine adjustments and concentrate on the experiment itself, measurements and result interpretation.
Due to the combination of the low-noise registration
system, unique scanner, advanced electronics and smart scanning procedures
that incorporated over 100 years of combined SPM research experience, with
AFM HERON one can perform unique measurements which are extremely
difficult, if possible at all, using other SPM instruments.
Extra-safe and at the same time fast landing procedure makes it possible to protect even very sharp tips from any possible damage. Due to the availability of the true non-contact scanning mode one can measure even the most fragile and mechanically sensitive samples. Unique smart scanning procedures allow to obtain high quality images on very challenging objects like 130 nm Ag nanoparticles or modern high density hard drive disks.
One of the unique features of HERON AFM is its
scanner. Due to our innovative design and flexure guide technology, this
scanner with built-in capacitive sensors shows the unmatched performance
characteristics (significant scanner natural frequencies come up to 20-30
kHz in XY and up to 35-40 kHz in Z) allowing a significant increase in the
scanning speed without sacrificing the image quality. The scanner allows
high scan rate imaging of samples with coarse topography features. The image
of the 540 nm calibration grating (Fig. 5) is obtained at 10Hz line
The advanced digital controller equipped with the optimized scanning control algorithms allows to decrease the phase lag, overshooting and ringing during the scanning process, making sure that the quality of the image remains the same even at higher speeds.
The novel proprietary MFM imaging mode allows the user to obtain the magnetic profile of the sample at outstanding speed. This MFM image of the magnetic structure of surface domains in Yttrium Iron Garnet (YIG) film was acquired at 10 Hz scan rate.
Figure 6. Magnetic structure of surface domains in Yttrium Iron Garnet (YIG) film.
Due to very well designed and calculated construction of
the AFM and the scanner, HERON AFM features outstanding
mechanical stability, which allows the user to get atomic resolution images
with the same 100 micron scanner and at the same time to produce high
quality images without vibration isolation tables. This is of extreme
importance for the integration of the AFM with the optical facilities on top
of an optical table.
DNA sample imaged with DLC supersharp cantilever.
1x1µm scan, 512 dpl, 1Hz scan rate.
Both single- and double- stranded DNAs are present on the sample. The twist structure of the double-stranded DNAs can be observed even in this relatively large scan.
DNA-Protein complex sample imaged with Si fpN01S cantilever.
1x1µm scan, 512 dpl, 1Hz scan rate. Roughness analysis along the line reveals that the surface of this sample is relatively rough, R3z being equal to 0.48 nm
DNA related presentations at SPIE Defense and Security Sensing
Where Del Mar Photonics product's names come from?
Del Mar means 'by the sea", and Photonics is all about optical waves. So we decided to choose terms popular in surfing and sailing communities. Our femtosecond lasers, amplifiers and systems are named after popular surf breaks around the world, and many other products names after sailing and nautical terms.