Del Mar Photonics - Plasmonic nano-materials for Raman spectroscopy for security and sensing applications - Single Molecule Raman spectra of Trinitrotoluene ( TNT)
Wellcome Trust Biocentre, University of Dundee
Department of Cell Biology, Harvard Medical School
12:30 - 1:05
Changing Decision Made by a Single Molecule Event: Deciphering a Genetic
Switch in a Living Bacterial Cell
1:05 - 1:40
imaging of protein distribution and dynamics
1:40 - 2:15
Carlton (speaker) & John Sedat, UCSF
Exploring chromosome structure with superresolution imaging methods
2:15 - 2:50
Schwille, BIOTEC, Dresden
||Fluorescence Correlation Spectroscopy in live and reconstituted cellular systems|
2:50 - 3:10
3:10 - 3:45
||One in one thousand: Capturing the dynamics of receptor oligomerization by single molecule tracking|
3:45 - 4:20
Tomography: A new tool for imaging tissue molecular architecture
4:45 - 5:20
molecule FRET and stepping behavior of motor proteins
Del Mar Photonics
we are looking for a CW dye laser for single molecule spectroscopy. We need linewidth < 1 MHz, longterm stability better than 100 kHz/second and a very good pointing stability.
"Optical Detection and Spectroscopy of Single Molecules in a Solid," by W. E. Moerner and L. Kador, Phys. Rev. Lett. 62, 2535 (1989). This is the first report of single-molecule detection and spectroscopy in condensed phases.
"Fluorescence Spectroscropy and Spectral Diffusion of Single Impurity Molecules in a Crystal," by W.P. Ambrose and W. E. Moerner, Nature 349, 225 (1991).
"Spectroscopy of Single Impurity Molecules in Solids," by W. E. Moerner and Th. Basche', Angew. Chem. 105, 537 (1993); Angew. Chem. Int. Ed. Engl. 32, 457 (1993).
"Examining Nanoenvironments in Solids on the Scale of a Single, Isolated Impurity Molecule," by W. E. Moerner, Science 265, 46 (1994).
"High-Resolution Optical Spectroscopy of Single Molecules in Solids, by W. E. Moerner, in "Single Molecules and Atoms," Special Issue of Accounts of Chemical Research, December 1996.
"Fundamentals of Single-Molecule Spectroscopy in Solids," Chapter 1 of Single Molecule Optical Detection, Imaging, and Spectroscopy, T. Basche, W. E. Moerner, M. Orrit, and U. P. Wild, eds. (Verlag Chemie, Munich, 1997).
W. E. Moerner, "Those Blinking Single Molecules," Science 277, 1059 (1997).
W. E. Moerner and M. Orrit, "Illuminating Single Molecules in Condensed Matter," Science 283, 1670-1676 (1999).
B. Lounis and W. E. Moerner, "Single Photons on Demand from a Single Molecule at Room Temperature," Nature 407, 491-493 (2000).
H. Sosa, E. J. G. Peterman, W. E. Moerner, and L. S. B. Goldstein, "ADP-Induced Rocking of the Kinesin Motor Domain Revealed by Single-Molecule Fluorescence Polarization Microscopy," Nature Structural Biology 8, 540-544 (2001).
W. E. Moerner, "Thirteen Years of Single-Molecule Spectroscopy in Physical Chemistry and Biophysics," in Single-Molecule Spectroscopy: Nobel Conference Lectures, R. Rigler, M. Orrit, Th. Basche, Editors, Springer Series in Chemical Physics, Volume 67 (Springer-Verlag, Heidelberg, 2001), pp. 32-61.
W. E. Moerner, "A Dozen Years of Single-Molecule Spectroscopy in Physics, Chemistry, and Biophysics," J. Phys. Chem. B 106, 910-927 (2002).
W. E. Moerner, "Single-Molecule Optical Spectroscopy of Autofluorescent Proteins," J. Chem. Phys. 117, 10925 (2002).
W. E. Moerner and D. P. Fromm, "Methods of Single-Molecule Fluorescence Spectroscopy and Microscopy," Rev. Sci. Instrum. 74, 3597-3619 (2003).
E. J. G. Peterman, H. Sosa, and W. E. Moerner, “Single-Molecule Fluorescence Spectroscopy and Microscopy of Biomolecular Motors,” invited review, Ann. Rev. Phys. Chem. 55, 79-96 (2004).
P. J. Schuck, D. P. Fromm, A. Sundaramurthy, G. S. Kino, and W. E. Moerner, “Improving the Mismatch Between Light and Nanoscale Objects with Gold Bowtie Nanoantennas,” Phys. Rev. Lett. 94, 017402 (2005).
K. A. Willets, S. Y. Nishimura, P. J. Schuck, R. J. Twieg, and W. E. Moerner, "Nonlinear Optical Chromophores as Nanoscale Emitters for Single-Molecule Spectroscopy," invited review, Accounts Chem. Res. 38, 549-556 (2005)
S. Y. Kim, Z. Gitai, A. Kinkhabwala, L. Shapiro, and W. E. Moerner, “Single Molecules of the Bacterial Actin MreB Undergo Directed Treadmilling Motion in Caulobacter crescentus,” Proc. Nat. Acad. Sci. (USA) 103, 10929-10934 (2006)..
A. E. Cohen and W. E. Moerner, “Suppressing Brownian Motion of Individual Biomolecules in Solution,” Proc. Nat. Acad. Sci. (USA) 103, 4362-4365 (2006).
W. E. Moerner, “New Directions in Single-Molecule Imaging and Analysis,” Invited Perspective, Proc. Nat. Acad. Sci. (USA) 104, 12596-12602 (2007).
J. S. Biteen, M. A. Thompson, N. K. Tselentis, G. R.Bowman, L. Shapiro, W. E. Moerner, “Superresolution Imaging in Live Caulobacter Crescentus Cells Using Photoswitchable EYFP,” Nature Meth. 5, 947-949 (2008).
W. E. Moerner, “Single-Molecule Optical Spectroscopy and Imaging: From Early Steps to Recent Advances,” in Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium 138, Springer Series in Chemical Physics Vol. 96, A. Gräslund, R. Rigler, J. Widengren, Eds. ( Springer-Verlag, Berlin, 2009).
A. Kinkhabwala, Z. Yu, S. Fan, Y. Avlasevich, K. Müllen, and W. E. Moerner, “Large Single-Molecule Fluorescence Enhancements Produced by a Bowtie Nanoantenna,” Nature Photonics 3, 654-657 (2009)..
S. J. Lord, H.-L. D. Lee, and W. E. Moerner, “Single-Molecule Spectroscopy and Imaging of Biomolecules in Living Cells," Anal. Chem. 82, 2192-2203 (2010) .
CW single-frequency ring Dye laser DYE-SF-077 -
Frequency-stabilized CW single-frequency ring Dye laser, model DYE-SF-077, is a further development of model DYE-SF-07. It now includes a system of frequency stabilization on the basis of a thermo-stabilized interferometer and a fast electronic driver.
Laser DYE-SF-077 features exceptionally narrow generation line width, which amounts to less than 100 kHz. DYE-SF-077 sets new standard for generation line width of commercial lasers. Prior to this model, the narrowest line-width of commercial dye lasers was as broad as 500 kHz - 1 MHz. It is necessary to note that the 100-kHz line-width is achieved in DYE-SF-077 without the use of an acousto-optical modulator, which, as a rule, complicates the design and introduces additional losses. A specially designed ultra-fast PZT is used for efficient suppression of radiation frequency fluctuations in a broad frequency range
570-700 nm, output > 1.5 W (10 W pump), linewidth < 100 kHz rms, frequency drift < 30 MHz/hour, smooth scanning 6/20 GHz.
The DYE-SF-077 laser cavity has horizontal orientation, the optical mounts of the cavity elements are attached to a rigid base plate, which is further stabilized by a volumetric framework with three invar rods underneath. Additional passive stability of the position of cavity elements is provided by the vibration isolating design of the laser base.
Dye laser DYE-SF-077 is the first representative of the new generation of dye lasers that offer to the user virtually the same level of convenience and simplicity of operation as with a solid-state tunable laser. As a result we are able to offer an option of combined configuration of DYE-SF-077 with Ti:Sapphire laser.
Laser DYE-SF-077 may be equipped with a USB compatible interface to remotely scan the generation line of the laser and to perform multi-channel data acquisition. Laser DYE-SF-077 also may be shipped together with an atom cell and a system for reduction of long-term generation line drift. Besides, laser DYE-SF-077 in combination with highly-efficient resonant frequency doubler FD-SF-07 delivers several hundreds milliwatts of narrow-band UV radiation within the 285–350-nm range.
CW single-frequency ring Dye laser DYE-SF-077 - request a quote
|Wavelength range|| 570-620 nm
|Output|| > 1 W at 6 W pump
>1.5W at 10W pump
|Linewidth||< 100 kHz rms1|
|Frequency drift||< 30 MHz/hour|
|Smooth scanning||> 6 GHz3|
1. relative to the reference cavity
2. < 1 MHz/hour with frequency stabilization to an atomic/molecular line (option)
3. up to 20 / 40 GHz (option)
1. 20 / 40 GHz smooth scanning;
2. 285-350 nm wavelength range with Resonant Frequency Doubler FD-SF-07
3. Absolute Frequency stabilization to an atomic/molecular line
4. + Ti:Sapphire laser (linewidth < 5 kHz) in the same Laser head
Dye Circulation System
CW single-frequency ring Dye laser DYE-SF-077 (Standard quotation) - request a quote
Actively frequency-stabilized, continuous-wave, single-frequency
ring Dye laser, model DYE-SF-077
Unique DYE-SF-077 laser has more narrow linewidth for Dye lasers on the present market. DYE-SF-077 laser has super-narrow linewidth (< 70 kHz) and unique Auto Re-lock function which is extremely useful in a work with frequency stabilized laser.
Installation of the T&D Scan high resolution Laser Spectrometer based on broadly tunable CW laser at the Drexel University
DYE-SF-077 datasheet (1,2 Mb)
Del Mar Photonics, Inc.
4119 Twilight Ridge
San Diego, CA 92130
tel: (858) 876-3133
fax: (858) 630-2376